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Table 4 Comparison of genes involved in pyruvate metabolism and C1 metabolism between T. saccharolyticum and its relative species

From: Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485

Organisms

Enzymes

PFOR

PDH

PFL

Glycine cleavage system

Lipoic acid synthesis

Lipoic salvage system

Clostridium thermocellum DSM1313

+

+

b

b

Clostridium clariflavum DSM 19732

+

+

b

b

Clostridium stercorarium subsp. stercorarium DSM 8532

+

+

+

+

Thermoanaerobacter saccharolyticum JW/SL-YS485

+

+

+

+

Thermoanaerobacter tengcongensis MB4(T)

+

+

+

+

+

Thermoanaerobacter sp. X514

+

+

+

+

+

Thermoanaerobacter pseudethanolicus ATCC 33223

+

+

+

+

+

Thermoanaerobacter italicus Ab9

+

+

+

+

Thermoanaerobacter mathranii subsp. mathranii A3

+

+

+

+

Thermoanaerobacter brockii subsp. finnii Ako-1

+

+

+

+

+

Thermoanaerobacter wiegelii Rt8.B1

+

+

+

+

+

Thermoanaerobacter kivui DSM 2030

+

+

+

+

Thermoanaerobacterium thermosaccharolyticum DSM571

+

+a

+

+

Thermoanaerobacterium xylanolyticum LX-11

+

+

+

+

Caldicellulosiruptor saccharolyticus DSM 8903

+

+

Caldicellulosiruptor bescii DSM 6725

+

+

Caldicellulosiruptor obsidiansis OB47

+

+

Caldicellulosiruptor hydrothermalis 108

+

+

Caldicellulosiruptor owensensis OL

+

+

Caldicellulosiruptor kristjanssonii 177R1B

+

b

b

Caldicellulosiruptor kronotskyensis 2002

+

+

Caldicellulosiruptor lactoaceticus 6A

+

b

b

  1. a T. thermosaccharolyticum DSM571 has pfl annotated, whereas T. thermosaccharolyticum M0795 does not have it. It is also confirmed with protein blast using PFL protein sequence from T. saccharolyticum.
  2. bNo information about lipoic acid metabolism of C. thermocellum DSM1313, C. clariflavum DSM 19732, C. kristjanssonii 177R1B and C. lactoaceticus 6A in KEGG. The existence of lipoic acid biosynthesis and lipoic salvage system are confirmed by protein blast using lipoyl synthase from C. bescii and lipoate protein ligase from T. saccharolyticum.