Table 6 Oligonucleotides used in this study
Primer | Target gene | Sequence (5′–3′) |
|---|---|---|
JP75 | Kanamycin cassette from pMU433 | TAAACCGCTAAGGCATGA |
JP76 | CTATCTGCATCGTCTTTTC | |
JP77 | pMU433 backbone | AGTTAGGATGTTGGCAGA |
JP78 | AAAGAGGGCATACAAGGA | |
JP209 | Erythromycin cassette from ALK2 | TGCAGGTCGATAAACCCAG |
JP210 | GAATTCCCTTTAGTAACGTGTAACTTTC | |
JP211 | Replication region from pUC19 | CATTAATGAATCGGCCAAC |
JP212 | CTCGTGATACGCCTATTT | |
JP143 | external to pforA cluster | GCTGTGGCAACTTAACAA |
JP144 | CTCATATCATCCGCTCCT | |
JP167 | external to pforB cluster | GTTGTTGTTTTGGCTTAGG |
JP168 | AGGCTTTCATTCAGTACG | |
JP169 | external to pforD cluster | CGTGCCTTTTGACCTTCC |
JP170 | CTGCTGTCTCGTCCTATT | |
JP171 | external to pforF cluster | CCAATATACCACCAGCCA |
JP172 | GAATTTAGGAAAACCGCCA | |
JP181 | external to pfl cluster | ATCCCTCTGTGTCTTTATC |
JP182 | TGGTTGTGGGTGTTTATG | |
recA-F | qPCR for Tsac_1846 (recA) | GAAGCCTTAGTGCGAAGTGG |
recA-R | GAAGTCCAACATGTGCATCG | |
pfor-F | qPCR for Tsac_0046 | ATCAAGCTTGGAATGGGTTG |
pfor-R | GCTGTTGGAGCCTTTGAGTC | |
pfl-F | qPCR for Tsac_0628 | CTATAGCATCGCCTGCTGTG |
pfl-R | TCGATACCGCCGTTTATAGC | |
pfl_ae-F | qPCR for Tsac_0629 | ATTGCCATAACCCTGACACA |
pfl_ae-R | TAGGCTCTCCACCTGTCAGC |