Fig. 1
Characterization of the moderately growing subisolate from a Botryococcus CCALA 779 culture. a Growth curve of the subisolate. X- and Y-axis indicate time in days and cell dry weight in gram per liter. Nutrient strength of 1× BB and 2× BB indicates medium with standard nutrient concentration and twofold of standard concentration. b Fluorescence microscopic analysis of Nile red-stained cells of subisolate. Cells prior to and after ND are indicated by +N and −N, respectively. Cell images are captured under the setting of differential interference contrast (DIC) and fluorescence (Nile red). A scale bar of 20 μm is shown. c Analysis of total lipids extracted by methanol/chloroform solution (i.e., 2:1 by volume). Equal amount of total lipid extract from cells prior to (+N) and after (−N) ND is loaded onto a TLC plate and developed with hexane/diethyl ether/acetic acid solution (i.e., 35:15:0.1 by volume). Origin and front of TLC are indicated. Marker (M) of standard chemicals includes squalene (SQU), beta-carotene (bCAR), FAME C14-C22 (FAM), tri-oleic acid glyceride (TAG), oleic acid (FFA), and mono-olein (MOL). HC hydrocarbon.