The subisolate belongs to the group of Botryococcus braunii race A. a Phylogenetic analysis of the subisolate with other Botryococcus spp. based on the 18S rDNA sequence. Groups of Botryococcus spp. race A, B, L are indicated. The subisolate of CCALA 779 in the phylogenetic tree is indicated. b GC spectrum of hydrocarbons extracted from cells of the subisolate prior to and after ND. X- and Y-axis indicate time in minute (min) and absorbance (abs) in arbitrary unit (a.u.). Compositions of hydrocarbon are indicated. The asterisk (*) indicates an unknown chemical species. c Mass spectra of the heptacosdiene C27H52 and heptacostriene C27H50. X- and Y-axis indicate mass in Dalton and density in arbitrary unit (a.u.), respectively.