Transcriptomic analysis of a moderately growing subisolate Botryococcus braunii 779 (Chlorophyta) in response to nitrogen deprivation

Table 1 Growth curves of the Botryococcus sp. subisolate

Time	CDW1^a	CDW2	CDW3	CDW (Ave ± SD)^b
(A) In BB medium
0 (d)	0.140	0.155	0.155	0.150 ± 0.009
2	0.215	0.245	0.235	0.232 ± 0.015
4	0.600	0.640	0.630	0.623 ± 0.021
6	0.920	0.960	0.970	0.950 ± 0.026
8	1.150	1.350	1.300	1.267 ± 0.104
10	1.500	1.700	1.650	1.617 ± 0.104
12	1.900	2.200	1.900	2.000 ± 0.173

Period	Rate1^c	Rate2	Rate3	Rate (Ave ± SD)
(A) In BB medium
0–2	0.239	0.257	0.231	0.243 ± 0.013
2–4	0.671	0.616	0.637	0.641 ± 0.027
4–6	0.238	0.225	0.241	0.235 ± 0.009
6–8	0.118	0.186	0.158	0.154 ± 0.034
Average growth rate	0.318
Maximum growth rate	0.641

Time	CDW1	CDW2	CDW3	CDW (Ave ± SD)
(B) In 2× BB medium
0 (d)	0.140	0.155	0.155	0.150 ± 0.009
2	0.730	0.755	0.750	0.745 ± 0.013
4	1.610	1.580	1.630	1.607 ± 0.025
6	2.250	2.500	2.550	2.433 ± 0.161
8	3.000	3.000	3.300	3.100 ± 0.173
10	3.500	3.850	3.850	3.733 ± 0.202
12	3.800	4.300	4.050	4.050 ± 0.250

Period	Rate1	Rate2	Rate3	Rate (Ave ± SD)
(B) In 2× BB medium
0–2	1.2835	1.2070	1.1997	1.2301 ± 0.0464
2–4	0.4851	0.4466	0.4742	0.4686 ± 0.0198
4–6	0.1822	0.2579	0.2508	0.2303 ± 0.0418
6–8	0.1547	0.0954	0.1376	0.1292 ± 0.0305
Average growth rate	0.515
Maximum growth rate	1.230

^aCDW stands for cell try weight, repeat number is indicated.
^bAve and SD stand for average and standard deviation based on 3 repeats.
^cGrowth rate in an 2 days-period of 0–2 days, 2–4 days, 4–6 days, and 6–8 days was calculated using the formula \( \sqrt[2]{CDW_{t2}/CDW_{t1}}- 1. \)

ISSN: 2731-3654