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Table 1 Growth curves of the Botryococcus sp. subisolate

From: Transcriptomic analysis of a moderately growing subisolate Botryococcus braunii 779 (Chlorophyta) in response to nitrogen deprivation

Time CDW1a CDW2 CDW3 CDW (Ave ± SD)b
(A) In BB medium
 0 (d) 0.140 0.155 0.155 0.150 ± 0.009
 2 0.215 0.245 0.235 0.232 ± 0.015
 4 0.600 0.640 0.630 0.623 ± 0.021
 6 0.920 0.960 0.970 0.950 ± 0.026
 8 1.150 1.350 1.300 1.267 ± 0.104
 10 1.500 1.700 1.650 1.617 ± 0.104
 12 1.900 2.200 1.900 2.000 ± 0.173
Period Rate1c Rate2 Rate3 Rate (Ave ± SD)
(A) In BB medium
 0–2 0.239 0.257 0.231 0.243 ± 0.013
 2–4 0.671 0.616 0.637 0.641 ± 0.027
 4–6 0.238 0.225 0.241 0.235 ± 0.009
 6–8 0.118 0.186 0.158 0.154 ± 0.034
Average growth rate 0.318    
Maximum growth rate 0.641    
Time CDW1 CDW2 CDW3 CDW (Ave ± SD)
(B) In 2× BB medium
 0 (d) 0.140 0.155 0.155 0.150 ± 0.009
 2 0.730 0.755 0.750 0.745 ± 0.013
 4 1.610 1.580 1.630 1.607 ± 0.025
 6 2.250 2.500 2.550 2.433 ± 0.161
 8 3.000 3.000 3.300 3.100 ± 0.173
 10 3.500 3.850 3.850 3.733 ± 0.202
 12 3.800 4.300 4.050 4.050 ± 0.250
Period Rate1 Rate2 Rate3 Rate (Ave ± SD)
(B) In 2× BB medium
 0–2 1.2835 1.2070 1.1997 1.2301 ± 0.0464
 2–4 0.4851 0.4466 0.4742 0.4686 ± 0.0198
 4–6 0.1822 0.2579 0.2508 0.2303 ± 0.0418
 6–8 0.1547 0.0954 0.1376 0.1292 ± 0.0305
Average growth rate 0.515    
Maximum growth rate 1.230    
  1. aCDW stands for cell try weight, repeat number is indicated.
  2. bAve and SD stand for average and standard deviation based on 3 repeats.
  3. cGrowth rate in an 2 days-period of 0–2 days, 2–4 days, 4–6 days, and 6–8 days was calculated using the formula \( \sqrt[2]{CDW_{t2}/CDW_{t1}}- 1. \)