Fig. 3

Sugar conversion rates from synergetic hydrolysis by extra-enzyme of C. owensensis and CTec2 on native corn stover (a) and native corncob (b). c, d are the HPLC lines of the hydrolysate at the end of hydrolysis (72 h) on native corn stover and native corncob respectively. SH (sequential hydrolysis): hydrolyzed by the enzyme of C. owensensis at 70 °C for 48 h (the xylan and glucan conversion rates after this hydrolysis were shown at 0 h in a, b) then adding CTec2 and incubating at 50 °C for 72 h. CH (co-hydrolysis): co-hydrolyzed by the enzyme of C. owensensis and CTec2 at 50 °C for 72 h. CTec2: hydrolyzed by CTec2 only as control. The loading rates of CTec2 for synergetic hydrolysis were 30 mg/g glucan (High loading). The loading rates of enzyme of C. owensensis for synergetic hydrolysis were 15 mg/g dry substrate. The amounts of released glucose and xylose were used for calculating glucan and xylan conversions, respectively