Characterization of hemicellulase and cellulase from the extremely thermophilic bacterium Caldicellulosiruptor owensensis and their potential application for bioconversion of lignocellulosic biomass without pretreatment

Table 3 Hydrolysis rates of lignocellulosic biomass by enzymes of C. owensensis

Substrate	Enzymes (15 mg/g ds)	Sugar released
Substrate	Enzymes (15 mg/g ds)	Xylose (% of xylan)	Arabinose (% of araban)	Glucose (% of glucan)	Reducing sugar (% of carbohydrate)^a
Native corn straw	Extra-enzyme	9.6 ± 0.32	20.9 ± 0.78	3.9 ± 0.14	15.4 ± 0.56
	Extra-/Intra- = 1:1	14.7 ± 0.56	39.5 ± 1.23	3.7 ± 0.14	14.3 ± 0.67
	Intra-enzyme	7.9 ± 0.33	53.5 ± 2.81	3.1 ± 0.12	11.5 ± 0.43
Native corncob	Extra-enzyme	11.7 ± 0.47	22.9 ± 1.12	ND	14.5 ± 0.61
	Extra-/Intra- = 1:1	16.8 ± 0.48	45.7 ± 1.73	ND	13.7 ± 0.57
	Intra-enzyme	10.4 ± 0.51	60.0 ± 2.85	ND	10.9 ± 0.49
SE corn stover	Extra-enzyme	50.0 ± 2.23	ND	ND	4.6 ± 0.17
	Extra-/Intra- = 1:1	59.1 ± 2.54	ND	ND	3.8 ± 0.16
	Intra-enzyme	36.4 ± 1.67	ND	ND	2.5 ± 0.12

ISSN: 1754-6834