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Table 3 Thioacidolysis results compared with lignin and S/G estimates using pyrolysis molecular beam mass spectrometry (PyMBMS)

From: Down-regulation of p-coumaroyl quinate/shikimate 3′-hydroxylase (C3′H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla × E. grandis leads to improved sugar release

Sample Thioacidolysis PyMBMS
% S % G % H S/G Ratio S/G Ratio Lignin
Control a 66.0 32.6 1.5 2.0 2.1 30.0
Control b 65.8 33.7 0.5 1.9 2.1 30.9
Control avg 65.9 33.2 1.0 2.0 2.1 30.4
C3′H 105 51.7 34.0 14.2 1.5 1.4 22.9
C3′H 106a 48.3 34.2 17.6 1.4 1.2 20.5
C3′H 106b 46.9 34.2 19.0 1.4 1.1 21.2
C3′H 113a 49.1 32.4 18.5 1.5 1.2 21.5
C3′H 113b 48.8 31.2 19.9 1.6 1.2 20.6
C3′H 114 46.1 33.5 20.4 1.4 1.1 19.6
C3′H avg 48.5* 33.2 18.3* 1.5* 1.2* 21.0*
C4H 73a 58.6 38.2 3.1 1.5 1.2 21.9
C4H 73b 59.0 40.2 0.9 1.5 1.2 21.9
C4H 91 57.7 41.4 0.9 1.4 1.3 23.1
C4H 92 58.2 41.2 0.7 1.4 1.3 22.5
C4H 93 59.2 39.7 1.1 1.5 1.2 21.1
C4H 96 58.4 40.8 0.8 1.4 1.2 21.8
C4H 98 61.5 37.6 0.9 1.6 1.3 22.9
C4H 99a 60.4 38.5 1.2 1.6 1.1 19.0
C4H 99b 58.0 40.9 1.0 1.4 1.1 21.2
C4H avg 59.0* 39.8* 1.2 1.5* 1.2* 21.7*
  1. * Indicates significant difference from the control average at the p value <0.05 level