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Table 3 Thioacidolysis results compared with lignin and S/G estimates using pyrolysis molecular beam mass spectrometry (PyMBMS)

From: Down-regulation of p-coumaroyl quinate/shikimate 3′-hydroxylase (C3′H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla × E. grandis leads to improved sugar release

Sample

Thioacidolysis

PyMBMS

% S

% G

% H

S/G Ratio

S/G Ratio

Lignin

Control a

66.0

32.6

1.5

2.0

2.1

30.0

Control b

65.8

33.7

0.5

1.9

2.1

30.9

Control avg

65.9

33.2

1.0

2.0

2.1

30.4

C3′H 105

51.7

34.0

14.2

1.5

1.4

22.9

C3′H 106a

48.3

34.2

17.6

1.4

1.2

20.5

C3′H 106b

46.9

34.2

19.0

1.4

1.1

21.2

C3′H 113a

49.1

32.4

18.5

1.5

1.2

21.5

C3′H 113b

48.8

31.2

19.9

1.6

1.2

20.6

C3′H 114

46.1

33.5

20.4

1.4

1.1

19.6

C3′H avg

48.5*

33.2

18.3*

1.5*

1.2*

21.0*

C4H 73a

58.6

38.2

3.1

1.5

1.2

21.9

C4H 73b

59.0

40.2

0.9

1.5

1.2

21.9

C4H 91

57.7

41.4

0.9

1.4

1.3

23.1

C4H 92

58.2

41.2

0.7

1.4

1.3

22.5

C4H 93

59.2

39.7

1.1

1.5

1.2

21.1

C4H 96

58.4

40.8

0.8

1.4

1.2

21.8

C4H 98

61.5

37.6

0.9

1.6

1.3

22.9

C4H 99a

60.4

38.5

1.2

1.6

1.1

19.0

C4H 99b

58.0

40.9

1.0

1.4

1.1

21.2

C4H avg

59.0*

39.8*

1.2

1.5*

1.2*

21.7*

  1. * Indicates significant difference from the control average at the p value <0.05 level
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Biotechnology for Biofuels and Bioproducts

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