Table 2 Strains, plasmids, and primers used in this study
Bacterial strain, plasmid or primer | Relevant characteristic or sequence | Source or restriction |
|---|---|---|
Strains | ||
 B. subtilis ACR | B. subtilis 168 with pMA5-HpaII-acr, source of yodC and ldhA genes | Lab stock |
 B. subtilis AY | B. subtilis ACR blocked in YodC by insertion of a Cat expression cassette | This study |
 B. subtilis AFY | B. subtilis ACR blocked in YodC by insertion of fdh gene | This study |
 B. subtilis AFYL | B. subtilis AFY blocked in LdhA by insertion of a CAT expression cassette | This study |
 E. coli JM109 | recA1, endA1, gyrA96, thi-1, hsd R17(rk- mk +)supE44 | Invitrogen |
 Candida boidinii | Source of fdh gene | Lab stock |
Plasmids | Â | Â |
 pMD18-T | Cloning vector | Takara |
 pBGSC6 | Containing Cat expression cassette | Lab stock |
 pMA5-HpaII | Expression vector (in E. coli, Apr; in B. subtilis, Kanr) | Lab stock |
 T-cat | pMD18-T with Cat expression cassette | This study |
 T-yodC | pMD18-T with yodC | This study |
 T-yodC::cat | cat inserted into the EcoR V site of T-yodC | This study |
 T-fdh | pMD18-T with fdh | This study |
pMA5-fdh | pMA5-HpaII with fdh | Â |
 T-HpaII-fdh | pMD18-T with HpaII-fdh | This study |
 T-yodC::fdh | fdh inserted into the Eco47 III site of T-yodC | This study |
 T-ldhA | pMD18-T with ldhA | This study |
 T-ldhA::cat | cat inserted into the EcoR V site of T-ldhA | This study |
Primers 5′-3′ |  |  |
 P1 | ACCGGGATCCATGACGAATACTCTGGAT | BamH I |
 P2 | ACCGACGCGTTTACAGCCAAGTTGATAC | Mlu I |
 P3 | CGCGATATCAAAAAAGGATTGATTCTAATG | EcoR V |
 P4 | CGCGATATCTAGTGACATTAGAAAACCGAC | EcoR V |
 P5 | CGCCATATGATGAAGATCGTTTTAGTC | Nde I |
 P6 | CGCACGCGTTTATTTCTTATCGTGTTTAC | Mlu I |
 P7 | ACCGAGCGCTATTTTTTGAGTGATCTTCTC | Eco47 III |
 P8 | ACCGAGCGCTTTATTTCTTATCGTGTTTAC | Eco47 III |
 P9 | ACCGGGATCCATGATGAACAAACATG | BamH I |
 P10 | ACCGACGCGTTTAGTTGACTTTTTGTTCTG | Mlu I |