Fig. 2
From: Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose
Construction of recombinant vectors for ALS and meso-BDH expression. a Construction of pETDuet–PT7–budB–PT7–budC. b Construction of pET28a–lysR–Pabc–budB–budC. lysR, the gene encoding the transcriptional regulator; budB, the gene encoding ALS; budA, the gene encoding ALDC; budC, the gene encoding meso-BDH, P abc , the predicted promoter of the 2,3-BD pathway gene cluster from E. cloacae subsp. dissolvens strain SDM