Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose

Table 2 Enzyme activities of E. coli strains harboring different vectors in 24 h flask cultures

Strain	ALS activity (U/mg)	ALDC activity (U/mg)	BDH activity (U/mg)
			Reduction		Oxidation
			Diacetyl	Acetoin	meso-2,3-BD
E. coli BL21 (pETDuet-1)	0.03 ± 0.04	ND	0.18 ± 0.01	0.20 ± 0.04	ND
E. coli BL21 (pET28a–lysR–P_abc–budB–budC)	0.16 ± 0.03	ND	1.79 ± 0.17	1.17 ± 0.09	0.38 ± 0.02
E. coli BL21 (pETDuet–P_T7–budB–P_T7–budC)	1.67 ± 0.06	ND	7.20 ± 1.21	4.20 ± 0.23	2.80 ± 0.20

Data are the mean ± standard deviations (SDs) from three parallel experiments
ALS α-acetolactate synthase, ALDC α-acetolactate decarboxylase, BDH butane-2,3-diol dehydrogenase

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