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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Modular design of metabolic network for robust production of n-butanol from galactose–glucose mixtures

Fig. 4

a Production of n-butanol by GAL_080 harboring pCOLADuet, GAL_081~084 harboring fdh1 variants after cultivation for 48 h in galactose-supplemented modified TB medium. b Time-course of fermentation profiles of the optimized strain (GAL_083), showing maximum n-butanol production. The pH was adjusted to around 7.2 at 6-h intervals. The left y-offset and right y-axis represent galactose and metabolite (n-butanol, ethanol, and butyrate) concentrations (g/L), respectively. The left y-axis represents OD600. The x-axis represents time in culture (h). The error bars indicate standard deviations of measurements from two independent cultures. Symbols: open circle OD600; open rectangle galactose; closed up triangle n-butanol; closed diamond ethanol; closed down triangle butyrate

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