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Table 1 Yeast strains and plasmids used in this study

From: Increasing proline and myo-inositol improves tolerance of Saccharomyces cerevisiae to the mixture of multiple lignocellulose-derived inhibitors

Yeast strains or plasmids

Description

References

S. cerevisiae strains

 BY4741

MATa HIS3 LEU2 MET15 URA3

Research genetics

 BY4742

MATα HIS3 LEU2 LYS2 URA3

Research genetics

 BY4742-13659 (ΔPRO1)

Isogenic to BY4742, except PRO1::KAN

Research genetics

 BY4742-11620 (ΔPRO2)

Isogenic to BY4742, except PRO2::KAN

Research genetics

 BY4742-11272 (ΔINO1)

Isogenic to BY4742, except INO1::KAN

Research genetics

 BY4742-13646 (ΔINM2)

Isogenic to BY4742, except INM2::KAN

Research genetics

 BY4742-10287 (ΔGLY1)

Isogenic to BY4742, except GLY1::KAN

Research genetics

 BY4742-15969 (ΔLYS1)

Isogenic to BY4742, except LYS1::KAN

Research genetics

 BY4742-13403 (ΔSHM1)

Isogenic to BY4742, except SHM1::KAN

Research genetics

 BY4742/PRO1

BY4742 (pRS426-HXT7p-PRO1-TEF1t)

This study

 BY4742/INO1

BY4742 (pRS426-HXT7p-INO1-TEF1t)

This study

 BY4742/pRS426

BY4742 (pRS426)

This study

 BY4742/PRO2

BY4742 (pRS426-HXT7p-PRO2-TEF1t)

This study

 BY4742/INM2

BY4742 (pRS426-HXT7p-INM2-TEF1t)

This study

Plasmid

 pRS426

URA3, no expression (control plasmid)

[45]

 pRS426-HXT7p-PRO1-TEF1t

URA3, pRS426 with PRO1 inserted between HXT7 promoter and TEF1 terminator

This study

 pRS426-HXT7p-INO1-TEF1t

URA3, pRS426 with INO1 inserted between HXT7 promoter and TEF1 terminator

This study

 pRS426-HXT7p-PRO2-TEF1t

URA3, pRS426 with PRO2 inserted between HXT7 promoter and TEF1 terminator

This study

 pRS426-HXT7p-INM2-TEF1t

URA3, pRS426 with INM2 inserted between HXT7 promoter and TEF1 terminator

This study