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Table 1 Yeast strains and plasmids used in this study

From: Increasing proline and myo-inositol improves tolerance of Saccharomyces cerevisiae to the mixture of multiple lignocellulose-derived inhibitors

Yeast strains or plasmids Description References
S. cerevisiae strains
 BY4741 MATa HIS3 LEU2 MET15 URA3 Research genetics
 BY4742 MATα HIS3 LEU2 LYS2 URA3 Research genetics
 BY4742-13659 (ΔPRO1) Isogenic to BY4742, except PRO1::KAN Research genetics
 BY4742-11620 (ΔPRO2) Isogenic to BY4742, except PRO2::KAN Research genetics
 BY4742-11272 (ΔINO1) Isogenic to BY4742, except INO1::KAN Research genetics
 BY4742-13646 (ΔINM2) Isogenic to BY4742, except INM2::KAN Research genetics
 BY4742-10287 (ΔGLY1) Isogenic to BY4742, except GLY1::KAN Research genetics
 BY4742-15969 (ΔLYS1) Isogenic to BY4742, except LYS1::KAN Research genetics
 BY4742-13403 (ΔSHM1) Isogenic to BY4742, except SHM1::KAN Research genetics
 BY4742/PRO1 BY4742 (pRS426-HXT7p-PRO1-TEF1t) This study
 BY4742/INO1 BY4742 (pRS426-HXT7p-INO1-TEF1t) This study
 BY4742/pRS426 BY4742 (pRS426) This study
 BY4742/PRO2 BY4742 (pRS426-HXT7p-PRO2-TEF1t) This study
 BY4742/INM2 BY4742 (pRS426-HXT7p-INM2-TEF1t) This study
Plasmid
 pRS426 URA3, no expression (control plasmid) [45]
 pRS426-HXT7p-PRO1-TEF1t URA3, pRS426 with PRO1 inserted between HXT7 promoter and TEF1 terminator This study
 pRS426-HXT7p-INO1-TEF1t URA3, pRS426 with INO1 inserted between HXT7 promoter and TEF1 terminator This study
 pRS426-HXT7p-PRO2-TEF1t URA3, pRS426 with PRO2 inserted between HXT7 promoter and TEF1 terminator This study
 pRS426-HXT7p-INM2-TEF1t URA3, pRS426 with INM2 inserted between HXT7 promoter and TEF1 terminator This study