A metabolomics-based strategy for identification of gene targets for phenotype improvement and its application to 1-butanol tolerance in Saccharomyces cerevisiae

Table 4 Potential 1-butanol tolerance-related metabolites identified from the OPLS model

Metabolite	High VIP	Large coeff	Correlation
2-Aminoadipic acid		✔	Positive
Aspartic acid		✔	Positive
Cadavarine	✔		Negative
Cystathionine		✔	Negative
Glutamic acid	✔	✔	Negative
Glutamine	✔		Negative
Isocitric acid + citric acid	✔		Negative
Threonine	✔	✔	Positive
Valine	✔		Negative

Italics indicates that the metabolite was subsequently considered for validation using metabolic enzyme deletion mutant(s)

ISSN: 2731-3654