Table 2 Evaluation of selective Z. mobilis recombinants with genes showing differential expressions in genome expression analysis
Inhibitors | Recombinants | Cell growth (OD600) | Glucose consumption (g/L) | Ethanol productivity (g/L/h) | Inhibitors conversion (%) |
|---|---|---|---|---|---|
4-Hydroxybenzaldehyde (H group) | ZM4::pHW20a | 0.51 ± 0.01 | 5.60 ± 0.99 | 0.22 ± 0.00 | 6.87 ± 0.25 |
ZM4::pHW20a-ZMO1116 | 0.53 ± 0.04 | 6.91 ± 0.38 | 0.25 ± 0.00 | 7.62 ± 0.68 | |
ZM4::pHW20a-ZMO1696 | 0.53 ± 0.01 | 6.72 ± 1.32 | 0.25 ± 0.00 | 16.04 ± 0.86 | |
ZM4::pHW20a-ZMO1885 | 0.54 ± 0.01 | 7.37 ± 0.05 | 0.26 ± 0.00 | 10.45 ± 0.94 | |
ZM4:pHW20a-gfp | 0.48 ± 0.01 | 6.64 ± 1.58 | 0.16 ± 0.18 | 7.52 ± 0.48 | |
ZM4:pHW20a-ZMO1288-gfp | 0.53 ± 0.04 | 10.68 ± 0.69 | 0.24 ± 0.00 | 14.05 ± 1.37 | |
Syringaldehyde (S group) | ZM4::pHW20a | 1.26 ± 0.03 | 16.58 ± 0.39 | 0.58 ± 0.01 | 8.37 ± 0.67 |
ZM4::pHW20a-ZMO1116 | 1.29 ± 0.02 | 16.98 ± 0.16 | 0.60 ± 0.01 | 9.91 ± 0.08 | |
ZM4::pHW20a-ZMO1696 | 1.33 ± 0.00 | 16.99 ± 0.06 | 0.57 ± 0.03 | 10.08 ± 1.09 | |
ZM4::pHW20a-ZMO1885 | 1.36 ± 0.00 | 17.02 ± 0.09 | 0.61 ± 0.02 | 10.92 ± 0.40 | |
ZM4:pHW20a-gfp | 1.29 ± 0.01 | 16.88 ± 0.02 | 0.60 ± 0.00 | 7.23 ± 0.38 | |
ZM4:pHW20a-ZMO1288-gfp | 1.30 ± 0.00 | 16.76 ± 0.05 | 0.60 ± 0.00 | 10.98 ± 0.83 | |
Vanillin (G group) | ZM4::pHW20a | 0.59 ± 0.03 | 12.62 ± 0.33 | 0.51 ± 0.24 | 5.44 ± 0.71 |
ZM4::pHW20a-ZMO1116 | 0.83 ± 0.07 | 14.68 ± 0.05 | 0.57 ± 0.00 | 6.50 ± 0.87 | |
ZM4::pHW20a-ZMO1696 | 0.64 ± 0.01 | 15.21 ± 0.37 | 0.55 ± 0.01 | 15.99 ± 1.13 | |
ZM4::pHW20a-ZMO1885 | 0.69 ± 0.01 | 13.65 ± 0.20 | 0.58 ± 0.01 | 10.74 ± 0.13 | |
ZM4::pHW20a-gfp | 0.61 ± 0.03 | 11.52 ± 1.58 | 0.42 ± 0.02 | 5.18 ± 0.59 | |
ZM4::pHW20a-ZMO1288-gfp | 0.89 ± 0.05 | 13.72 ± 0.25 | 0.48 ± 0.01 | 16.07 ± 0.07 |