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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Engineering Yarrowia lipolytica to produce biodiesel from raw starch

Fig. 6

Lipid production in lipid overproducer background. a, b Show the strain expressing alpha-amylase and glucoamylase in the lipid accumulating background JMY3820; the growth in purple (OD600), the percentage of fatty acids in the DCW (%FA) in blue and the citric acid produced (g/L) in green. Two culture media were used as described in “Methods”; YNBCN60 (C/N 60) and YNBCN90 (C/N 90). c, d Show fluorescence microscopy images where the lipid bodies were stained with Bodipy. c Corresponds to the experiment A while d corresponds to the experiment B. All presented data are the average of at least two independent experiments. The panels show representative cells that have been enlarged (×2)

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