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Table 5 Oligonucleotides used in this study

From: High production of 2,3-butanediol from biodiesel-derived crude glycerol by metabolically engineered Klebsiella oxytoca M1

Oligonucleotides

Sequencea

Source

pduCUp1

5′-TTATGCTTCTTTTTTACGCAGCTTATCG-3′

This study

pduCUp2

5′-TTTCTAGAGAATAGGAACTTC

GGGGAACTGCATCATGGAA-3′

This study

pduCUDFCF1

5′-TTCCATGATGCAGTTCCCC

GAAGTTCCTATTCTCTAGAAA-3′

This study

pduCUDFCF2

5′-GGCACAATTTTTTAATCTTA

GAAGTTCCTATACTTTCTAGA-3′

This study

pduCDown1

5′-TCTAGAAAGTATAGGAACTTC

TAAGATTAAAAAATTGTGCC-3′

This study

pduCDown2

5′-AATAAGCCTCAGAAAATTGAGTTA

GAAATAAAGTTGA-3′

This study

pduCcon1

5′-CCGTTTTCAACCAGCGTCAG-3′

This study

pduCcon2

5′-CCGGTAATTCTCACCCGGAG-3′

This study

ldhADown1

5′-TTACCAGACCACGGATTGCG-3′

This study

ldhADown2

5′-CTTTCTAGAGAATAGGAACTTC

CTTTCCCTTTTGTGCTCCT-3′

This study

ldhAUDFCF1

5′-GGAGCACAAAAGGGAAAG

GAAGTTCCTATTCTCTAGAAAG-3′

This study

ldhAUDFCF2

5′-TATTATCACTGGAGAAAAGTCTT

GAAGTTCCTATACTTTCTAGAGAA-3′

This study

ldhAUP1

5′-TTCTCTAGAAAGTATAGGAACTTC

AAGACTTTTCTCCAGTGATAATA-3′

This study

ldhAUP2

5′-GCGGGCTTTCATTGAGTGAG-3′

This study

ldhAcon1

5′-GGGAATTGTAACTTTATCGCAGGC-3′

This study

ldhAcon2

5′-CCGTGACGGTATTATCACTGGA-3′

This study

  1. aUnderlined sequences are homologous with the FRT region