Fig. 5
Evaluation of alterations in the composition of the photosynthetic apparatus of selected mutant strains. Western blot quantifications of LHCf1 (a) and LHCX1 (b), two major antenna proteins of the Nannochloropsis gaditana photosynthetic apparatus. As a control, the PSII core subunit D2 was also detected on the same membrane. Equivalents of 4 and 8 × 106 cells were loaded for the detection of LHCf1 and LHCX1, respectively. c Representative traces of fluorescence induction kinetics for WT and E2 DCMU-treated cells. On the 4th day of growth, 200 × 106 cells in late exponential phase were used for fluorescence measurements following excitation with 320 µmol photons m−2 s−1 of actinic light at 630 nm; d Fluorescence induction curve t 2/3 values were used to calculate the size of the PSII functional antenna. The data are expressed as the mean ± SD, n = 4; values that significantly differ from wild-type are marked with an asterisk (ANOVA, p value <0.05)