Fig. 3
Metabolic flux maps of xylose-to-ethanol fermentation for CBS4435 (a) and BP000 (b) together with a collection of specific enzyme activities (c) and intracellular metabolites (d) obtained for CBS4435 (hatched boxes) and BP000 (grey boxes). a, b External metabolites are underlined. Relative fluxes normalized to q xylose (0.67 mmol/gdc/h for CBS4435 and of 0.47 mmol/gdc/h for BP000) are shown. ATP and CO2 formation were used individually as objective function; resultant fluxes were averaged and presented together with their standard deviations. c Specific enzyme activities determined at 25 °C are shown in µmol/min/mgprotein. d Intracellular metabolites are shown in µmol/gdc. EC energy charge