Fig. 6

Results from predictability analysis. a, b Display comparisons of predicted to experimentally observed effects on q _xylose and Y _xylitol, respectively, relative to a reference state due to different network modification (see main text). Numbering used: 1 and 1′ fivefold and tenfold lower activity levels of PGI; 2, 4, 5, and 6 knockout, 20-fold and fivefold lower and 5.6-fold higher activity levels of G6PDH; 3 knockout of GND; 7′ (6.7–10)-fold higher activity levels of XR; 8′ and 9′ 29-fold and 72-fold higher activity levels of XDH. Data 7, 8 and 9 display corresponding effects after increasing the activity level of UG by a factor of 6 (XR: tenfold higher), 4 and 6, respectively. Solid lines indicate perfect match of simulated vs. experimentally obtained effects. To simulate knockouts, a specific activity of 0.001 µmol/min/mg⁻¹ was assumed (a value <0.01 µmol/min/mg has been reported [18]). Error bars indicate reported and obtained, by in silico analysis (±10 % variation on the extent of activity level modification was assumed), standard deviations