Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

Table 1 Characteristics of the integrative plasmids used in this study

Plasmid	Relevant features	References
pRDH225	KanMX, expression of T. reesei EGII gene	This study
pRDH226	ZeoR, expression of T. emersonii CBHI gene	This study
pIL2GA-SS	LEU2, display of R. oryzae glucoamylase	[50]
pIU5GA-SS	URA3, display of R. oryzae glucoamylase	[50]
pIBG-SS	HIS3, display of A. aculeatus BGL1	[22]
pIL2-EG_D	LEU2, display of T. reesei EGII	This study
pIL2-EG_S	LEU2, secretion of T. reesei EGII	This study
pIU5-CBHI_D	URA3, display of T. emersonii CBHI	This study
pIU5-CBHI_S	URA3, secretion of T. emersonii CBHI	This study

R. oryzae, Rhizopus oryzae; A. aculeatus, Aspergillus aculeatus; T. reesei, Trichoderma reesei; T. emersonii, Talaromyces emersonii; BGL1, β-glucosidase 1; EGII, endoglucanase II; CBHI, cellobiohydrolase I

ISSN: 2731-3654