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Table 1 Strain and plasmids used in this study

From: Cellulosic ethanol production via consolidated bioprocessing at 75 °C by engineered Caldicellulosiruptor bescii

Strains Strain and genotype/phenotype Source
Escherichia coli
 JW338 DH5α containing pDCW180 (ApramycinR) This study
 JW341 DH5α containing pDCW183 (ApramycinR) This study
Caldicellulosiruptor bescii
 JWCB001 wild type/(ura+/5-FOAS) DSMZa
 JWCB017 ΔpyrFAΔldh/(ura/5-FOAR) [49]
 JWCB049 ΔpyrFA Δldh CIS1:: PS-layerTeth39_0206 (adhE)/(ura/5-FOAR) This study
 JWCB054 ΔpyrFA Δldh CIS1:: PS-layerTeth39_0218 (adhB)/(ura/5-FOAR) This study
Plasmids
 pDCW142 Intermediate vector (ApramycinR) [26]
 pDCW180 Integration vector containing PS-layerTeth39_0206 (ApramycinR) This study
 pDCW183 Integration vector containing PS-layerTeth39_0218 (ApramycinR) This study
  1. aDeutsche Sammlung von Mikroorganismen und Zellkulturen