Table 1 Strain and plasmids used in this study

Escherichia coli

 JW338

DH5α containing pDCW180 (Apramycin^R)

This study

 JW341

DH5α containing pDCW183 (Apramycin^R)

This study

Caldicellulosiruptor bescii

 JWCB001

wild type/(ura⁺/5-FOA^S)

DSMZ^a

 JWCB017

ΔpyrFAΔldh/(ura⁻/5-FOA^R)

[49]

 JWCB049

ΔpyrFA Δldh CIS1:: P_S-layerTeth39_0206 (adhE)/(ura⁻/5-FOA^R)

This study

 JWCB054

ΔpyrFA Δldh CIS1:: P_S-layerTeth39_0218 (adhB)/(ura⁻/5-FOA^R)

This study

Plasmids

 pDCW142

Intermediate vector (Apramycin^R)

[26]

 pDCW180

Integration vector containing P_S-layerTeth39_0206 (Apramycin^R)

This study

 pDCW183

Integration vector containing P_S-layerTeth39_0218 (Apramycin^R)

This study