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Table 2 Carbon balance of fermentation products after 48 h fermentation

From: Cellulosic ethanol production via consolidated bioprocessing at 75 °C by engineered Caldicellulosiruptor bescii

Strains Concentration of residual compounds (at 48 h), mM Product yieds, mol/mol
Cellobiose initial Cellobiose final Glucose Cellobiose catablized Lactate Acetate Ethanol Hydrogen CR (%) Y L/C Y A/C Y E/C Y H/C
JWCB017 33.1 ± 0.42 19.7 ± 0.03 17.4 ± 0.19 4.7 ± 0.27 0 17.1 ± 0.32 0.0 23.9 ± 0.15 91 0 3.64 0 5.08
JWCB049 33.1 ± 0.05 19.6 ± 0.21 16.5 ± 0.07 5.3 ± 0.12 0 14.2 ± 0.08 2.9 ± 0.09 22.5 ± 0.39 81 0 2.67 0.54 4.25
JWCB054 33.4 ± 0.14 20.5 ± 0.07 16.2 ± 0.05 4.8 ± 0.05 0 15.5 ± 0.14 1.4 ± 0.09 15.5 ± 0.77 88 0 3.23 0.28 4.83
  1. Substrate and product concentration of wild-type, JWCB017, JWCB049 and JWCB054 are reported in mM with average percent carbon recovery (CR) and average yields in mol/mol. The fermentation conditions were as described in “Methods”. Product yields are calculated as: Y L/C lactate yield per mole cellobiose, Y A/C acetate yield per mole cellobiose, Y E/C ethanol yield per mole cellobiose, Y H/C hydrogen yield per mole cellobiose