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Table 2 Carbon balance of fermentation products after 48 h fermentation

From: Cellulosic ethanol production via consolidated bioprocessing at 75 °C by engineered Caldicellulosiruptor bescii

Strains

Concentration of residual compounds (at 48 h), mM

Product yieds, mol/mol

Cellobiose initial

Cellobiose final

Glucose

Cellobiose catablized

Lactate

Acetate

Ethanol

Hydrogen

CR (%)

Y L/C

Y A/C

Y E/C

Y H/C

JWCB017

33.1 ± 0.42

19.7 ± 0.03

17.4 ± 0.19

4.7 ± 0.27

0

17.1 ± 0.32

0.0

23.9 ± 0.15

91

0

3.64

0

5.08

JWCB049

33.1 ± 0.05

19.6 ± 0.21

16.5 ± 0.07

5.3 ± 0.12

0

14.2 ± 0.08

2.9 ± 0.09

22.5 ± 0.39

81

0

2.67

0.54

4.25

JWCB054

33.4 ± 0.14

20.5 ± 0.07

16.2 ± 0.05

4.8 ± 0.05

0

15.5 ± 0.14

1.4 ± 0.09

15.5 ± 0.77

88

0

3.23

0.28

4.83

  1. Substrate and product concentration of wild-type, JWCB017, JWCB049 and JWCB054 are reported in mM with average percent carbon recovery (CR) and average yields in mol/mol. The fermentation conditions were as described in “Methods”. Product yields are calculated as: Y L/C lactate yield per mole cellobiose, Y A/C acetate yield per mole cellobiose, Y E/C ethanol yield per mole cellobiose, Y H/C hydrogen yield per mole cellobiose