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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: An integrative Raman microscopy-based workflow for rapid in situ analysis of microalgal lipid bodies

Fig. 2

Raman spectra of fatty acid standards and microalgal lipids a green excitation (532 nm laser) and b NIR excitation (785 nm laser). Raman spectra are listed with decreasing degree of unsaturation of fatty acid standards from top to bottom and show a gradual decrease in the intensity of Raman band at 1650 rel cm−1 (C=C stretching mode) compared to that of 1440 rel cm−1 band (CH2 bending mode). Raman peaks in the 2800–3000 rel cm−1 (CH2 symmetric and asymmetric stretches) are scaled in intensity to allow visualization. These modes are more intense than the peaks at 1440 and 1650 rel cm−1 when using the green excitation and much weaker than the peaks at 1440 and 1650 rel cm−1 when using the NIR excitation

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