Table 4 Advantage and disadvantage of AC vs. NAC methods
Methods | Autoclaved biomass (AC method) | Non-autoclaved biomass, with antibiotics (NAC method) |
|---|---|---|
Operation time | Same | Same |
Control of contamination | Completely | Variable (depending on the feedstocks) |
Quality of hydrolysate (glucose and lactate) | Very similar | Variable |
Concentration of inhibitors | Most are higher than non-autoclaved ones | Most are lower than autoclaved ones |
Useful for yeast studies | Yes | Yes |
Useful for Zymomonas studies | Yes | Variable (depending on strains and antibiotics used) |
Industrial SOP | No | Probable |
Environmental issues | None | Development of antibiotics resistance |