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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: An engineered cryptic Hxt11 sugar transporter facilitates glucose–xylose co-consumption in Saccharomyces cerevisiae

Fig. 2

Characterization of the N366X mutants of HXT11. a Growth of the hexokinase deficient DS71054 strain containing the empty vector (filled square), or expression vectors bearing HXT11 (open circle), and HXT11-N366D (filled circle) on 1 % xylose and 15 % glucose. b Uptake of [14C-] xylose by the DS68625 strain expressing HXT11 (open circle) and HXT11-N366D (filled circle) in the presence of increasing concentrations of glucose. c Detailed view of the sugar-binding pocket of the Hxt11 homology model, showing the first shell amino acid side chains that interact with bound glucose (cyan) and xylose (yellow). N366 is located to the left, pointing the side chain towards the 6-OH and 6-CH2 of glucose. Most residues in this pocket are strictly conserved between Hxt11 and XylE, apart from D337 (I in XylE), A442 (G in XylE), Y446 (W in XylE) and N469 (Q in XylE). The scheme was constructed using Maestro (Schrödinger LLC, NY, USA). d Exponential growth rates (μ) of strain DS71054 expressing the indicated Hxt11-N366X mutants when grown on 10 % glucose and 1 % xylose. The black bar indicated the wild-type position, and the gray bar indicates the N366D mutant obtained in the error-prone mutagenesis. The dashed line indicates the growth rate of strain DS71054 without any introduced transporter. The error bars are the standard error of the mean from two technical samples

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