Skip to main content

Table 1 Strains and plasmids

From: An engineered cryptic Hxt11 sugar transporter facilitates glucose–xylose co-consumption in Saccharomyces cerevisiae

Strain/plasmid

Relevant genotype and/or characteristics

Source or reference

S. cerevisiae strains

 DS68616

Mat a, ura3-52, leu2-112, gre3::loxP, loxP-Ptpi:TAL1, loxP-Ptpi::RKI1, loxP-Ptpi-TKL1, loxP-Ptpi-RPE1, delta::Padh1XKS1Tcyc1-LEU2, delta::URA3-Ptpi-xylA-Tcyc1, delta::his3

This paper

 DS68625

DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP

This paper

 DS68625-evo

DS68625-derivative after evolutionary engineering

This paper

 DS71054

DS71055, glk1::lox72; hxk1::loxP; hxk2::lox72; gal1::loxP; his3::loxPnatMXloxP

This paper

 DS71055

DS68616-derivative after evolutionary engineering

DSM, The Netherlands

Plasmids

 pRS313

E. coli/yeast shuttle vector; CEN6, ARSH4, HIS3, Ampr

[27]