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Table 2 Simultaneous and sequential reactions of Ac-Abf51A and XynBE18 against water-soluble wheat arabinoxylan

From: A novel bifunctional GH51 exo-α-l-arabinofuranosidase/endo-xylanase from Alicyclobacillus sp. A4 with significant biomass-degrading capacity

Enzyme added Amounts of xylooligosaccharides (mg/mL) Amounts of arabinose (mg/mL) Degree of synergya
First reaction Second reaction Xylose Xylobiose Xylotriose
XynBE18 None 0.26 ± 0.01 0.45 ± 0.02
Ac-Abf51A None 0.004 ± 0.00 0.006 ± 0.00 0.14 ± 0.01
Ac-Abf51A XynBE18 0.30 ± 0.01 0.63 ± 0.03 0.14 ± 0.01 1.26
XynBE18 Ac-Abf51A 0.21 ± 0.01 0.61 ± 0.02 0.20 ± 0.01 0.77 ± 0.03 2.12
XynBE18 + Ac-Abf51A None 0.62 ± 0.03 1.14 ± 0.04 0.72 ± 0.04 2.92
  1. The amounts of xylooligosaccharides and arabinose were determined by HPAEC
  2. –, not detected
  3. aDegree of synergy is defined as the ratio of the amounts of saccharide released from simultaneous or sequential enzyme combinations to the sum of that released by the individual enzymes