Fig. 2

Quantitative titration and optimization of the PDCzm-slr1192 pathway based on the in vitro reconstitution system. a In slr1192 titration system (black), PDCzm concentrations were fixed at 0.05 μΜ, while in PDCzm titration system (red), slr1192 concentrations were fixed at 0.05 μΜ. b Total protein concentrations (closed) and unit activities (open) dynamics of the PDCzm titration (circle) and slr1192 titration (square) systems. c Regulation of PDCzm and slr1192 ratios in the in vitro reconstitution systems with fixed total protein concentrations (10 mg/l). d Titration of the optimized (PDCzm-slr1192) unit with a concentration ratio of 4:6. The single unit indicated a concentration ratio of 4:6 between PDCzm and slr1192, while serial concentrations of such a unit were added to the in vitro system. e Titration of NADPH as cofactor in the optimized in vitro system. f Titration of pyruvate as substrate in the optimized in vitro system