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Table 1 In vitro titration fit values of PDCzm, slr1192, NADPH, pyruvate, TPP, Mg2+ ion and acetaldehyde

From: Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories

Components

K 50 /K m

V max (μM/s)

PDCzm

0.326 ± 0.071 μM

2.074 ± 0.120

slr1192

0.109 ± 0.014 μM

1.722 ± 0.045

NADPH

0.136 ± 0.013 mM

2.211 ± 0.085

Pyruvate

6.496 ± 0.505 mM

2.064 ± 0.104

TPP

0.011 ± 0.002 mM

1.405 ± 0.054

Mg2+

0.104 ± 0.017 mM

1.329 ± 0.043

Acetaldehyde

0.393 ± 0.130 mM

3.538 ± 0.228

  1. PDCzm and slr1192 titrations were performed in an in vitro system containing 10 mM pyruvates, 0.2 mM NADPH, 10 mM MgCl2, 0.1 mM TPP, 0.05 μM PDCzm or slr1192. NADPH, pyruvate, TPP, Mg2+ and acetaldehyde titrations were performed in an optimized system containing 10 mg/l PDCzm-slr1192 with a concentration ratio of 4:6
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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