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Table 1 Activities of iLDHs in crude cell extracts of P. putida KT2440 and P. putida. KT2440 (∆lldR) cultured with different growth substrates

From: A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source

Growth substrate Strain Enzyme activity (nmol/min mg protein)a
l-iLDH d-iLDH
dl-Lactate P. putida KT2440 161.4 ± 4.6 332.9 ± 4.3
P. putida KT2440 (∆lldR) 220.9 ± 7.0 471.5 ± 3.5
Glycerol P. putida KT2440 ND ND
P. putida KT2440 (∆lldR) 348.2 ± 11.2 771.0 ± 9.0
  1. ND not detected
  2. aActivities of d-iLDH and l-iLDH were examined with 20 mM d-lactate or 20 mM l-lactate. DCIP was used as the electron acceptor. Results are mean ± SD of three parallel replicates