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Table 1 Activities of iLDHs in crude cell extracts of P. putida KT2440 and P. putida. KT2440 (∆lldR) cultured with different growth substrates

From: A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source

Growth substrate

Strain

Enzyme activity (nmol/min mg protein)a

l-iLDH

d-iLDH

dl-Lactate

P. putida KT2440

161.4 ± 4.6

332.9 ± 4.3

P. putida KT2440 (∆lldR)

220.9 ± 7.0

471.5 ± 3.5

Glycerol

P. putida KT2440

ND

ND

P. putida KT2440 (∆lldR)

348.2 ± 11.2

771.0 ± 9.0

  1. ND not detected
  2. aActivities of d-iLDH and l-iLDH were examined with 20 mM d-lactate or 20 mM l-lactate. DCIP was used as the electron acceptor. Results are mean ± SD of three parallel replicates