A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source

Table 2 Comparison of pyruvate productions by whole cells of P. putida KT2440, P. putida/pBSPPc_Gm-vgb, P. putida KT2440 (ΔlldR), and P. putida KT2440 (ΔlldR)/pBSPPc_Gm-vgb

Strain	Pyruvate (mM)	Yield (%)^a	Productivity (mmol/g DCW h)
KT2440	0	0	0
KT2440/pBSPPc_Gm-vgb	0	0	0
KT2440 (ΔlldR)	50.60 ± 0.38	50.9	0.81 ± 0.005
KT2440 (ΔlldR)/pBSPPc_Gm-vgb	90.85 ± 0.75	91.9	1.43 ± 0.002

The initial dl-lactate concentration was 100 mM. The biocatalysis reactions were conducted at 30 °C in phosphate buffer (pH 7.4) for 6 h with 10.5 g DCW/L of biocatalysts prepared from glycerol. Results are mean ± SD of three parallel replicates
^aThe yields of pyruvate were calculated based on the actual initial concentrations of dl-lactate measured by HPLC

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