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Table 2 Comparison of pyruvate productions by whole cells of P. putida KT2440, P. putida/pBSPPcGm-vgb, P. putida KT2440 (ΔlldR), and P. putida KT2440 (ΔlldR)/pBSPPcGm-vgb

From: A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source

Strain Pyruvate (mM) Yield (%)a Productivity (mmol/g DCW h)
KT2440 0 0 0
KT2440/pBSPPcGm-vgb 0 0 0
KT2440 (ΔlldR) 50.60 ± 0.38 50.9 0.81 ± 0.005
KT2440 (ΔlldR)/pBSPPcGm-vgb 90.85 ± 0.75 91.9 1.43 ± 0.002
  1. The initial dl-lactate concentration was 100 mM. The biocatalysis reactions were conducted at 30 °C in phosphate buffer (pH 7.4) for 6 h with 10.5 g DCW/L of biocatalysts prepared from glycerol. Results are mean ± SD of three parallel replicates
  2. aThe yields of pyruvate were calculated based on the actual initial concentrations of dl-lactate measured by HPLC