Fig. 2
From: Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica
Expression profiles of ylGAL genes in Y. lipolytica W29. Cells were first growing for 16 h in YNB medium with 1.0 % of glucose and then incubated for 3–24 h in YNB medium containing the indicated sugar at 0.1 or 1.0 %. End-point RT-PCR was performed after 3 h incubation in glucose, mannose, or galactose (a). Kinetics of ylGAL genes expression was monitored by quantitative RT-PCR in Y. lipolytica W29 incubated in YNB medium containing 1.0 % glucose (b), 1.0 % galactose (c), or a mixture of 1.0 % glucose and 1.0 % galactose (d). Gene expression levels were normalized based on the expression levels of the actin gene (ΔC T). In inoculum