Fig. 6
From: Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica
Functional analysis of Leloir pathway overexpression in Y. lipolytica and fold change in the expression of the ylGAL genes. Growth of different Y. lipolytica transformants overexpressing ylGAL and scGAL genes on plates of YNB medium containing glucose or galactose—incubation at 28 °C, 48 h (a). Fold changes in the expression of ylGAL genes in the quadruple mutant, Y4588 (in which all the Leloir genes were overexpressed), relative to Y. lipolytica W29 (the wild-type strain) (b); yeast were incubated for 3 h in YNB medium containing 0.1 or 1.0 % galactose. Gene expression levels were normalized based on the expression of the actin gene