Fig. 1

Restoration of amino acid catabolism in native ‘decarboxylase-negative’ background by 2-oxo acid decarboxylase expression. Relative specific growth rates in micro-titre plate (µ_MTP) of IME140 (PDC1 PDC5 PDC6 ARO10) (white bars), IME259 (pdc1Δ, pdc5Δ, pdc6Δ, aro10Δ, MTHΔT, p426GPD) (black bars), IME260 (pdc1Δ, pdc5Δ, pdc6Δ, aro10Δ, MTHΔT, ARO10↑) (grey bars), IME261 (pdc1Δ, pdc5Δ, pdc6Δ, aro10Δ, MTHΔT, kdcA↑) (blue bars) and IME262 (pdc1Δ, pdc5Δ, pdc6Δ, aro10Δ, MTHΔT, kivD↑) (red bars) in SMG supplemented with 5 g/L (NH₄)₂SO₄ (NH₄ ⁺), valine (Val), leucine (Leu), isoleucine (Ile), phenylalanine (Phe) and methionine (Met). Cells were grown aerobically in 500 µL volumes in 48-well plates and incubated at 30 °C with OD 660 measured at 15 min intervals. Data are presented as averages and standard deviations of duplicate experiments relative to the average specific growth rate in micro-titre plate of IME140 in each nitrogen source. Specific growth rates in micro-titre plate for IME140 on each nitrogen source were as follows; NH₄ ⁺: 0.33 ± 0.01 h⁻¹, Val: 0.24 ± 0.01 h⁻¹, Leu: 0.16 ± 0.00 h⁻¹, Ile: 0.14 ± 0.00 h⁻¹, Phe: 0.19 ± 0.00 h⁻¹, Met: 0.18 ± 0.00 h⁻¹. NG: No Growth