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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae

Fig. 1

Restoration of amino acid catabolism in native ‘decarboxylase-negative’ background by 2-oxo acid decarboxylase expression. Relative specific growth rates in micro-titre plate (µMTP) of IME140 (PDC1 PDC5 PDC6 ARO10) (white bars), IME259 (pdc1Δ, pdc5Δ, pdc6Δ, aro10Δ, MTHΔT, p426GPD) (black bars), IME260 (pdc1Δ, pdc5Δ, pdc6Δ, aro10Δ, MTHΔT, ARO10↑) (grey bars), IME261 (pdc1Δ, pdc5Δ, pdc6Δ, aro10Δ, MTHΔT, kdcA↑) (blue bars) and IME262 (pdc1Δ, pdc5Δ, pdc6Δ, aro10Δ, MTHΔT, kivD↑) (red bars) in SMG supplemented with 5 g/L (NH4)2SO4 (NH4 +), valine (Val), leucine (Leu), isoleucine (Ile), phenylalanine (Phe) and methionine (Met). Cells were grown aerobically in 500 µL volumes in 48-well plates and incubated at 30 °C with OD 660 measured at 15 min intervals. Data are presented as averages and standard deviations of duplicate experiments relative to the average specific growth rate in micro-titre plate of IME140 in each nitrogen source. Specific growth rates in micro-titre plate for IME140 on each nitrogen source were as follows; NH4 +: 0.33 ± 0.01 h−1, Val: 0.24 ± 0.01 h−1, Leu: 0.16 ± 0.00 h−1, Ile: 0.14 ± 0.00 h−1, Phe: 0.19 ± 0.00 h−1, Met: 0.18 ± 0.00 h−1. NG: No Growth

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