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Table 2 2-oxo acid decarboxylase activities in cell extracts of S. cerevisiae strains expressing single 2-oxo acid decarboxylases, grown on different nitrogen sources

From: Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae

Substrate Strain Nitrogen source
NH4 + Phenylalanine Valine
Pyruvate IME259 (control) BD NG NG
IME260 (ARO10↑) BD BD NG
IME261 (kdcA↑) 0.013 ± 0.001 0.036 ± 0.006 0.033 ± 0.005
IME262 (kivD↑) BD 0.012 ± 0.003 BD
Phenylpyruvate IME259 (control) BD NG NG
IME260 (ARO10↑) BD 0.046 ± 0.001 NG
IME261 (kdcA↑) 0.045 ± 0.008 0.184 ± 0.029 0.155 ± 0.037
IME262 (kivD↑) BD 0.385 ± 0.040 0.013 ± 0.000
α-ketoisovalerate IME259 (control) BD NG NG
IME260 (ARO10↑) BD 0.056 ± 0.001 NG
IME261 (kdcA↑) 0.683 ± 0.146 2.509 ± 0.509 1.900 ± 0.153
IME262 (kivD↑) 0.093 ± 0.016 2.885 ± 0.107 0.151 ± 0.030
  1. Enzyme activities, expressed as U mg protein−1, were determined at the following substrate concentrations: pyruvate: 50 mM, phenylpyruvate: 12.5 mM, α-ketoisovalerate: 25 mM
  2. NG no growth, BD below detection limit of 0.008 U mg protein−1