Table 3 α-ketoisovalerate bioconversion under micro-aerobic conditions by S. cerevisiae strains expressing different 2-oxo acid decarboxylase genes
Strain | Decarboxylase | Biomass-specific production/consumption rates (mmol/g biomass/h) | |||||||
|---|---|---|---|---|---|---|---|---|---|
Glucose | Ethanol | KIV | Isobutanol | ||||||
pH 6.0 | pH 3.5 | pH 6.0 | pH 3.5 | pH 6.0 | pH 3.5 | pH 6.0 | pH 3.5 | ||
IME259 | None | 0.13 ± 0.01 | 0.15 ± 0.01 | BD | BD | 0.01 ± 0.00 | 0.04 ± 0.00 | BD | BD |
IME260 | ARO10↑ | 0.16 ± 0.04 | 0.19 ± 0.00 | 0.02 ± 0.01 | 0.02 ± 0.01 | 0.04 ± 0.02 | 0.10 ± 0.00 | 0.04 ± 0.00 | 0.05 ± 0.00 |
IME261 | kdcA↑ | 0.35 ± 0.03 | 0.35 ± 0.00 | 0.24 ± 0.00 | 0.20 ± 0.03 | 0.07 ± 0.00 | 0.17 ± 0.00 | 0.05 ± 0.00 | 0.10 ± 0.00 |
IME262 | kivD↑ | 0.22 ± 0.01 | 0.22 ± 0.01 | 0.10 ± 0.01 | 0.10 ± 0.00 | 0.06 ± 0.01 | 0.12 ± 0.00 | 0.03 ± 0.00 | 0.07 ± 0.01 |
IME140 | Wild-type control | 2.04 ± 0.25 | 1.71 ± 0.04 | 2.50 ± 0.05 | 2.86 ± 0.08 | 0.08 ± 0.01 | 0.26 ± 0.03 | 0.03 ± 0.01 | 0.10 ± 0.00 |