Skip to main content

Advertisement

Table 3 α-ketoisovalerate bioconversion under micro-aerobic conditions by S. cerevisiae strains expressing different 2-oxo acid decarboxylase genes

From: Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae

Strain Decarboxylase Biomass-specific production/consumption rates (mmol/g biomass/h)
Glucose Ethanol KIV Isobutanol
pH 6.0 pH 3.5 pH 6.0 pH 3.5 pH 6.0 pH 3.5 pH 6.0 pH 3.5
IME259 None 0.13 ± 0.01 0.15 ± 0.01 BD BD 0.01 ± 0.00 0.04 ± 0.00 BD BD
IME260 ARO10 0.16 ± 0.04 0.19 ± 0.00 0.02 ± 0.01 0.02 ± 0.01 0.04 ± 0.02 0.10 ± 0.00 0.04 ± 0.00 0.05 ± 0.00
IME261 kdcA 0.35 ± 0.03 0.35 ± 0.00 0.24 ± 0.00 0.20 ± 0.03 0.07 ± 0.00 0.17 ± 0.00 0.05 ± 0.00 0.10 ± 0.00
IME262 kivD 0.22 ± 0.01 0.22 ± 0.01 0.10 ± 0.01 0.10 ± 0.00 0.06 ± 0.01 0.12 ± 0.00 0.03 ± 0.00 0.07 ± 0.01
IME140 Wild-type control 2.04 ± 0.25 1.71 ± 0.04 2.50 ± 0.05 2.86 ± 0.08 0.08 ± 0.01 0.26 ± 0.03 0.03 ± 0.01 0.10 ± 0.00
  1. Biomass-specific conversion rates were measured after addition of 10 g/L glucose and 100 mM α-ketoisovalerate (KIV) to cell suspensions at pH 6.0 and pH 3.5. Cells were incubated micro-aerobically (see “Methods”) and incubated at 30 °C. Data are presented as averages and mean deviations of duplicate experiments
  2. BD below detection limit of HPLC