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Table 4 Estimated in vivo activities of 2-oxo acid decarboxylases during α-ketoisovalerate (KIV) bioconversion experiments

From: Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae

Strain Decarboxylase Substrate
Pyruvate KIV
IME259 None BD BD
IME260 ARO10 0.71 ± 0.29 2.02 ± 0.02
IME261 kdcA 8.07 ± 1.29 4.05 ± 0.05
IME262 kivD 3.74 ± 0.07 2.81 ± 0.38
IME140 Wild-type control 114 ± 3 3.83 ± 0.14
  1. Pyruvate and KIV decarboxylase activities were estimated from ethanol and isobutanol production rates at pH 3.5, based on a biomass protein content of 42 % [23]. Activity is expressed in mU mg protein−1
  2. BD below detection limit of HPLC