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Table 5 Saccharomyces cerevisiae strains used in this study

From: Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae

Strain Relevant genotype Origin
IME140 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 + p426GPD (2 µm URA3) [31, 49]
CEN.PK707-4A MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::loxP [13]
IMI244 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::loxP MTH1∆T This study
IMI271 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 ade2::PDC1_amdS This study
IMI275 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::loxP MTH1∆T ade2::PDC1_amdS This study
IMI302 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::cas9-tagA-loxP-natNT2-loxP MTH1∆T ade2::PDC1_amdS This study
IMK647 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::cas9-tagA-loxP-natNT2-loxP MTH1∆T aro10Δ This study
IME259 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::cas9-tagA-loxP-natNT2-loxP MTH1∆T aro10Δ p426GPD This study
IME260 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::cas9-tagA-loxP-natNT2-loxP MTH1∆T aro10Δ pUDE001 This study
IME261 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::cas9-tagA-loxP-natNT2-loxP MTH1∆T aro10Δ pUDE321 This study
IME262 MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1::loxP pdc5::loxP pdc6::cas9-tagA-loxP-natNT2-loxP MTH1∆T aro10Δ pUDE336 This study
CEN.PK113-7D MATa URA3 HIS3 LEU2 TRP1 MAL2-8c SUC2 [31, 32]
CEN.PK113-5D MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 [31, 32]
TAM MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 pdc1Δ::loxP pdc5Δ::loxP pdc6Δ::loxP, selected for C2 independence in glucose-limited chemostat cultures and glucose-tolerant growth in batch culture [39]