Table 6 Plasmids used in this study

pUD342

AmpR, E. coli replicon, COkdcA

This study

pUD350

AmpR, E. coli replicon, COkivD

This study

p426GPD

2 µm ori, URA3, TDH3 _p -CYC1 _t

[36]

pUDE001

2 µm ori, URA3, TDH3p-ARO10-CYC1t

[14]

pUDE321

2 µm ori, URA3, TDH3p-kdcA-CYC1t

This study

pUDE336

2 µm ori, URA3, TDH3p-kivD-CYC1t

This study

pUG-AmdS

2 µm ori, URA3, TEF2p-amdS-TEF2t

[40]

pUG-natNT2

2 µm ori, URA3, TEF2p-natNT2-TEF2t

[45]

p414-pTEF1-Cas9-tCYC1

2 µm ori, URA3, TEF1p-cas9-CYC1t

[44]

pROS10

2 μm URA3 gRNA-CAN1.Y gRNA-ADE2.Y

[43]