Bioaugmentation of Lactobacillus delbrueckii ssp. bulgaricus TISTR 895 to enhance bio-hydrogen production of Rhodobacter sphaeroides KKU-PS5

Table 1 Cumulative hydrogen production, hydrogen production rate (HPR), and hydrogen yield (HY) at different lactic acid-producing bacteria/purple non-sulfur photosynthetic bacteria (LAB/PNSB) ratios and initial cell concentration

Condition	LAB/PNSB ratio (w/w)	Initial cell conc. (g/L)	LAB conc. (g/L)	PNSB conc. (g/L)	Cumulative H₂ production (ml H₂/L)	HPR (ml H₂/L h)	HY (mol H₂/mol glucose)
A
A1	1:1	0.05	0.025	0.025	0	0	0
A2		0.10	0.050	0.050	0	0	0
A3		0.15	0.075	0.075	0	0	0
A4		0.20	0.100	0.100	0	0	0
A5		0.25	0.125	0.125	0	0	0
B
B1	1:2	0.05	0.017	0.033	1355 ± 113	2.1 ± 0.2	3.01 ± 0.25
B2		0.10	0.033	0.067	721 ± 65	1.3 ± 0.1	1.58 ± 0.11
B3		0.15	0.050	0.100	0	0	0
B4		0.20	0.067	0.133	0	0	0
B5		0.25	0.083	0.167	0	0	0
C
C1	1:7	0.05	0.066	0.044	1662 ± 41	3.1 ± 0.1	4.84 ± 0.11
C2		0.10	0.013	0.088	1460 ± 107	2.9 ± 0.2	4.00 ± 0.34
C3		0.15	0.019	0.131	1336 ± 56	2.7 ± 0.2	3.65 ± 0.25
C4		0.20	0.025	0.175	1403 ± 153	2.6 ± 0.3	3.39 ± 0.41
C5		0.25	0.031	0.219	1359 ± 129	2.6 ± 0.2	3.12 ± 0.20
D
D1	1:12	0.05	0.004	0.046	1613 ± 69	3.1 ± 0.1	4.44 ± 0.19
D2		0.10	0.008	0.092	1590 ± 38	3.0 ± 0.1	4.47 ± 0.11
D3		0.15	0.012	0.138	1833 ± 72	3.6 ± 0.3	5.93 ± 0.23
D4		0.20	0.015	0.185	1587 ± 86	3.6 ± 0.2	5.84 ± 0.30
D5		0.25	0.019	0.231	1573 ± 96	3.6 ± 0.2	5.82 ± 0.32

ISSN: 2731-3654