Table 5 Hydrogen production rate (HPR) and hydrogen yield (HY) by different bioaugmentation experiments
Augmented microorganisms | Native microorganisms | Substrate | HPR | HY | Ref. |
|---|---|---|---|---|---|
Enterobacter cloacae (DSM 16657) Escherichia Coli XL1-BLUE Mix consortium Non-augmentation | Anaerobic-enriched mixed cultures (EMC) from compost of food waste | Beverage wastewater (10 g glucose equivalent/L) | 93.8 ml H2/L h 51.0 ml H2/L h 70.1 ml H2/L h 75.6 ml H2/L h | 0.84a mol H2/mol glucose 0.63a mol H2/mol glucose 0.70a mol H2/mol glucose 1.13a mol H2/mol glucose | [23] |
Bacillus subtilis, Pseudomonas stutzeri, Lysinibacillus fusiformis, non-augmentation | Anaerobic consortium collected from a full scale anaerobic reactor treating composite wastewater | Real-field food wastewater (50 g COD/L) | 52.1a ml H2/L h 19.8a ml H2/L h 24.8a ml H2/L h 5.0a ml H2/L h | 3.8a mol H2/kg COD 1.4a mol H2/kg COD 1.9a mol H2/kg COD 0.4a mol H2/kg COD | [16] |
Clostridium sp. TCW1 semicontinuous culture fed three times daily Before augmentation | Enriched cellulolytic bacteria (Bacillus thermoamylovorans 9-4AIA and Clostridium thermocellum DSM 1313) | Vegetable-based kitchen waste and Napier grass (20 g COD/m3 day) | 4.6a ml H2/L h 2.1a ml H2/L h | 14 mol H2/kg CODin 6 mol H2/kg CODin | [17] |
Buttiauxella sp. 4, Rahnella sp. 10, Raoultella sp. 47, Mix consortium Non-augmentation | Indigenous microbial communities in vegetable waste | Vegetable waste | 60a ml H2/L h 64a ml H2/L h 60a ml H2/L h 100a ml H2/L h 24a ml H2/L h | 71.3 ml H2/g VS 47.5 ml H2/g VS 69.7 ml H2/g VS 85.7 ml H2/g VS 21.9 ml H2/g VS | [18] |
Ethanoigenens harbinense B49 Non-augmentation (strain X9 only) | Clostridium acetobutylicum X9 | Dark fermentation of microcrystal-line cellulose (10 g/L) | – | 1810 ml H2/L 755 ml H2/L | [20] |
Selectively enriched kanamycin-resistant mixed consortia | Native anaerobic mixed microflora in chemical wastewater | Chemical wastewater treatment | 0.02 mol H2/kg COD h | – | [21] |
Non-augmentation | 0.012 mol H2/kg COD h | ||||
Ethanoligenens sp. B49 Non-augmentation | Activated sludge | – (OLR 12 kg/m3.d) | 0.07a ml H2/L h 0.04a ml H2/L h | – | [22] |
Lactobacillus delbrueckii ssp. balgaricus TISTR 895 Non-augmentation (strain KKU-PS5 only) Strain TISTR 895 under optimal condition | Rhodobacter sphaeroides KKU-PS5 | Glucose (5 g/L) | 3.6 ± 0.3 ml H2/L h 3.2 ± 0.1 ml H2/L h 9.1 ± 0.2 ml H2/L h | 5.93 ± 0.23 mol H2/mol glucose 4.38 ± 0.20 mol H2/mol glucose 9.65 ± 0.23 mol H2/mol glucose | This study |