Fig. 7

Neutral oligosaccharide inhibition on pure substrate enzymatic hydrolysis with or without monomeric sugar inhibition. Clusters labelled with A–G represented different pooled fractions after SEC separation. Avicel was treated with Ctec2 for glucose conversion; beechwood xylan was treated with Htec2, MP and Htec2 + MP 1:1 for xylose conversion. Microplate enzymatic hydrolysis condition: pure substrates (Avicel and beechwood xylan) were added to 1.25 % solids loading 500 μL reaction volume. Enzyme loading was 10 mg/g substrate with 24 h hydrolysis at 50 °C, 10 rpm and pH 4.8. Oligosaccharide concentration was at 10 g/L; monomeric sugars were at 20 g/L glucose, 10 g/L xylose. a, c the glucose and xylose conversion of substrates with neutral oligosaccharides inhibition; b, d the glucose and xylose conversion of substrates with neutral oligosaccharides inhibition and monomeric sugar inhibition