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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Single-cell screening of photosynthetic growth and lactate production by cyanobacteria

Fig. 2

Viability in droplets and sorting. a Growth of encapsulated Synechocystis cells represented by photomicrographs, scale bar 20 μm (top), and counted cells per droplet (graphs show mean ± SEM) for strains SAA005 (middle; n = 5 independent experiments) and SAW035 (bottom; n = 3). b Lactate production in strain SAA005 with and without a 12-h pre-incubation in darkness. Red vertical lines are added as visual reference (n = 30,000 droplets analyzed per time point, y-axis is broken). Insets at 7 h show re-binning of data in the x-axis interval 0.05–0.13. This is to visualize the difference between synchronization and no synchronization. c Histogram of lactate production for a mix of wild type, SAA005, and SAW035. d 12:1 model library of SAA005 and SAW035 shown as fluorescence from lactate (left) and enrichment in sorting (right)

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