Genotyping PCR results of D9 mutant and WT D.
tertiolecta. The template for lanes
1, 2, 3, 4, and 5: D9 mutant, wild type, negative control (without template), 1 kb ladder, positive control (using the reconstructed plasmid as the template), respectively. The primer pair for lanes
1, 2, 3, and 5: ble_F + ble_R (the predicted size is 503 bp). The two arrows indicate a non-specific binding band and the target bleomycin band. ble_F: AAGCTGACCAGCGCCGTTC, ble_R: CCACGAAGTGCACGCAGTT