Engineering a novel glucose-tolerant β-glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasse at high glucose concentration

Table 2 The kinetic parameters of Bgl6 and the mutants using 10 % cellobiose (w/v) as substrate

Enzymes	Optimal temperature	Optimal pH	K _m (mM)	K _cat (s⁻¹)	K _cat/K _m(s⁻¹ mM⁻¹)
Bgl6	50–55	6.0	38.45 ± 1.51	21.50 ± 0.38	0.56
V174A	55–60	6.0	315.39 ± 10.87	35.13 ± 2.46	0.11
V174C	60	5.5	45.07 ± 0.50	48.45 ± 0.39	1.07
A404 V	55	6.5	50.52 ± 2.51	31.97 ± 1.13	0.63
L441F	55	5.5	25.91 ± 2.02	14.73 ± 1.54	0.57
M3	60	5.5	49.19 ± 1.75	83.11 ± 4.12	1.69

The hydrolysis of cellobiose results in two molecules of glucose
The K _cat and K _m values were determined on the basis of the Michaelis–Menten equation

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