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Table 2 The kinetic parameters of Bgl6 and the mutants using 10 % cellobiose (w/v) as substrate

From: Engineering a novel glucose-tolerant β-glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasse at high glucose concentration

Enzymes Optimal temperature Optimal pH K m (mM) K cat (s−1) K cat/K m(s−1 mM−1)
Bgl6 50–55 6.0 38.45 ± 1.51 21.50 ± 0.38 0.56
V174A 55–60 6.0 315.39 ± 10.87 35.13 ± 2.46 0.11
V174C 60 5.5 45.07 ± 0.50 48.45 ± 0.39 1.07
A404 V 55 6.5 50.52 ± 2.51 31.97 ± 1.13 0.63
L441F 55 5.5 25.91 ± 2.02 14.73 ± 1.54 0.57
M3 60 5.5 49.19 ± 1.75 83.11 ± 4.12 1.69
  1. The hydrolysis of cellobiose results in two molecules of glucose
  2. The K cat and K m values were determined on the basis of the Michaelis–Menten equation