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Table 1 Bacterial strains, genetic elements, plasmids and primers used in this study

From: Utilising the native plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC6803 as a cloning site for enhanced product production

Strain Genotype/phenotype Source
TOP10 E. coli strain. F, mcrA0, ∆(mrr-hsdRMS-mcrBC), φ80dlacZ58(M15), ∆lacX74, recA1, araD139, ∆(araA-leu)7697, galU , galK0, rpsL (StrR), endA1, nupG Bio-Sciences, Dun Laoghaire, Dublin, Ireland
AA314 Wild-type Synechocystis PCC6803 strain K. Hellingwerf, UvA, Amsterdam, The Netherlands
UL006 PCC6803 transconjugant with Ptrc-YFP, psbA2 locus, KanR This study
UL007 PCC6803 transconjugant with Ptrc-YFP-E*, psbA2 locus, KanR This study
UL008 PCC6803 transconjugant with PpsbA2-YFP, psbA2 locus, KanR This study
UL018 PCC6803 transconjugant with pCA2.4-Ptrc-YFP, KanR This study
UL022 PCC6803 transconjugant with Ptrc-GAL, psbA2 locus, KanR This study
UL023 PCC6803 transconjugant with Ptrc-GAL-E*, psbA2 locus, KanR This study
Plasmid/ICE Genotype/phenotype Source
pEYFP YFP source plasmid Clontech, CA 94043, USA
pSF-OXB20-BetaGal β-galactosidase gene source plasmid Oxford Genetics Ltd, Oxfordshire, OX25 5HD, UK
ICE R391 Kanamycin resistance gene source Integrative conjugative element [26]
pUC18 AmR backbone plasmid Sigma-Aldrich, Arklow, Wicklow, Ireland
pCR2.1-pTRC-riboswitchE* pTRC-E* and pTRC source plasmid Eurofins mwg/operon, 85560 Ebersberg, Germany
PpsbA2-YFP PpsbA2 promoter, YFP, psbA2 integration site, KanR This study
Ptrc-YFP Ptrc promoter, YFP, psbA2 integration site, KanR This study
Ptrc-YFP-E* Ptrc promoter, YFP, riboswitch E*, psbA2 integration site, KanR This study
pCA2.4-Ptrc-YFP Ptrc promoter, YFP, pCA2.4 integration site, KanR This study
PpsbA2-GAL PpsbA2 promoter, β-galactosidase, psbA2 integration site, KanR This study
Ptrc-GAL Ptrc promoter, β-galactosidase, psbA2 integration site, KanR This study
Ptrc-GAL-E* Ptrc promoter, β-galactosidase, riboswitch E*, psbA2 integration site, KanR This study