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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Effects of overexpression of a bHLH transcription factor on biomass and lipid production in Nannochloropsis salina

Fig. 2

Expression of NsbHLH2 in transformants under different culture conditions. a qRT-PCR of transgenic NsbHLH2 mRNA by using qbH1 and qbH2 primers, where qbH1 is located in the vector. b qRT-PCR of transgenic and endogenous NsbHLH2 mRNA by using qbH3 and qbH4 primers. c Western blotting of FLAG-tagged NsbHLH2. The expected size of FLAG-tagged NsbHLH2 was 65 kD. AtpB [expected sizes of 72.6 kD (F-type H-ATPase ß subunit) and 53.13 kD (CF1 ß subunit of ATP synthase)] was used as a loading control. Accession number of CF1 ß subunit of ATP synthase from N. salina CCMP537 is YP_008519835; accession number of F-type H-ATPase ß subunit from N. gaditana B-31 is EWM25142. Homologs of these proteins were present in N. salina CCMP1776, and appeared to be good loading controls with constant expression level under different culture conditions. WT wild type; N normal conditions; NL nitrogen limitation; O osmotic stress. The data points represent the average of samples and error bars indicate standard error (n = 3). Significant differences, as determined by Student’s t test, are indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001)

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