Skip to main content

Advertisement

Table 2 Specific growth rate, biomass productivity, and FAME content of WT and two NsbHLH2 transformants

From: Effects of overexpression of a bHLH transcription factor on biomass and lipid production in Nannochloropsis salina

Culture condition Strain Specific growth rate (day−1)a Biomass productivity (mg/L/day) FAME content (%)
   Day 0–4b Day 4–8c Day 8–12d 8 day 12 day 8 day 12 day
Normal WT 0.43 ± 0.04 0.47 ± 0.03 0.15 ± 0.01 89.6 ± 8.8 168.8 ± 10.4 15.6 ± 0.8 21.7 ± 0.9
3–6 0.59 ± 0.03** 0.35 ± 0.04 0.11 ± 0.02 121.9 ± 11.5* 176.0 ± 9.7 16.5 ± 0.7 24.5 ± 1.2
3–11 0.67 ± 0.03** 0.32 ± 0.03 0.10 ± 0.03 133.3 ± 23.0 170.8 ± 9.9 16.5 ± 1.1 24.1 ± 1.6
N limitation WT 0.40 ± 0.04 0.36 ± 0.36   78.1 ± 2.4   42.3 ± 1.4  
3–6 0.64 ± 0.02** 0.12 ± 0.01   96.3 ± 5.4*   46.0 ± 3.3  
3–11 0.60 ± 0.03** 0.14 ± 0.02   93.7 ± 6.3*   41.5 ± 1.2  
Osmotic stress WT 0.38 ± 0.04 0.48 ± 0.03 0.21 ± 0.04 102.1 ± 12.3 168.7 ± 12.1 17.2 ± 0.9 30.1 ± 3.0
3–6 0.46 ± 0.04 0.47 ± 0.05 0.11 ± 0.02 126.0 ± 15.9 180.1 ± 4.9 17.8 ± 1.2 32.5 ± 3.8
3–11 0.46 ± 0.02 0.38 ± 0.01 0.14 ± 0.02 131.2 ± 19.6 153.1 ± 4.6 17.2 ± 0.4 29.9 ± 4.7
  1. The data points represent the average of samples and error bars indicate standard error (n = 4). Significant differences, as determined by Student’s t test, are indicated by asterisks (* P < 0.05, ** P < 0.01, *** P < 0.001)
  2. aSpecific growth rate was calculated as (μ/day) = ln(X 2/X 1)/(t 2 − t 1); where X 1 and X 2 are the initial and final biomass and t 1 and t 2 are the initial and final times
  3. bSpecific growth rate based on cell density on day 0 and day 4
  4. cSpecific growth rate based on cell density on day 4 and day 8
  5. dSpecific growth rate based on cell density on day 8 and day 12