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Table 2 Plant biomass-degrading enzymes found in the RWS metasecretome

From: Metasecretome analysis of a lignocellulolytic microbial consortium grown on wheat straw, xylan and xylose

Protein number CAZy family Action on (activity)a Best PSI-BLAST hit vs. NCBI (organism source) Accession no. COV (%) e value Identity (%) COV2 #P
RWS1 CBM48|GH13|CBM41 Alpha-glucan polysaccharides (alpha-amylase/pullulanase) Pullulanase (Klebsiella) EHS90708.1 100 3E−45 98 43 5
RWS2 CBM48|GH13|CBM41 Pullulanase (Klebsiella) WP_017144955.1 100 8E−48 100 37 3
RWS3 CBM48|GH13|CBM41 Pullulanase (Klebsiella) EHS90708.1 100 9E−34 97 33 3
RWS4 CBM48|GH13|CBM41 Pullulanase (Klebsiella) EHS90708.1 100 4E−41 96 28 3
RWS5 CBM48|GH13|CBM41 Pullulanase (Klebsiella) EHS90708.1 100 2E−64 99 45 3
RWS6 CBM48|GH13|CBM41 Pullulanase (Klebsiella) EHS90707.1 100 1E−91 98 34 4
RWS7 CBM48|GH13|CBM41 Pullulanase (Klebsiella) WP_017144955.1 100 2E−41 97 48 5
RWS8 CBM48|GH13|CBM41 Pullulanase (Klebsiella) EHS90708.1 100 2E−100 95 30 3
RWS9 GH10 Hemicellulose (endoxylanase) XynB19 precursor (Sphingobacterium) ACX30647.1 100 9E−47 95 57 5
RWS10 GH10 Endo-1,4-beta-xylanase precursor (Sphingobacterium) ACX30652.1 100 4E−67 95 38 3
RWS11 GH10 Endo-1,4-beta-xylanase precursor (Sphingobacterium) ACX30652.1 98 1E−101 92 27 5
RWS12 GH12 Cellulose (endoglucanase) Peptidase M16 (Sphingobacterium) WP_045752104.1 100 3E−96 97 17 2
RWS13 GH16 (Hemi)cellulose (endoglucanase/endogalactanase) Glycerophosphodiester phosphodiesterase (Sphingobacterium) WP_031286917.1 100 2E−70 99 55 5
RWS14 GH16 Glycerophosphoryl diester phosphodiesterase (Sphingobacterium) KKX48537.1 100 7E−50 100 34 3
RWS15 GH16 Glycerophosphoryl diester phosphodiesterase (Sphingobacterium) KKX48537.1 100 5E−77 100 24 3
RWS16 GH16 Glycerophosphodiester phosphodiesterase (Sphingobacterium) WP_031286917.1 100 2E−95 99 41 5
RWS17 GH3 Cellulose (beta-glucosidase) Beta-glucosidase (Sphingobacterium) CDS95530.1 100 7E−106 95 21 3
RWS18 GH3 Glycoside hydrolase family 3 (Sphingobacterium) KKX49060.1 81 1E−52 92 27 2
RWS19 GH3 Glycoside hydrolase family 3 (Sphingobacterium) KKX49060.1 100 9E−94 92 21 3
RWS20 GH3 Glycoside hydrolase family 3 (Sphingobacterium) KKX49060.1 75 3E−34 96 36 2
RWS21 GH43 Hemicellulose (beta-xylosidase/alpha-arabinofuranosidase) Aldose epimerase (Sphingobacterium) WP_045756400.1 100 4E−81 81 11 2
RWS22 GH43 GH43D19 precursor (Sphingobacterium) ACX30655.1 100 3E−83 98 29 3
RWS23 GH43 GH43D19 precursor (Sphingobacterium) ACX30655.1 100 1E−51 100 37 2
RWS24 GH43 GH43D19 precursor (Sphingobacterium) ACX30655.1 97 8E−40 94 38 2
RWS25 GH43 GH43A19 precursor (Sphingobacterium) ACX30649.1 100 3E−88 98 15 2
RWS26 GH51 Hemicellulose (alpha-arabinofuranosidase) Alpha-l-arabinofuranosidase (Sphingobacterium) WP_045756399.1 100 1E−88 99 27 2
RWS27 GH51 Alpha-l-arabinofuranosidase (Sphingobacterium) WP_045756399.1 92 5E−45 99 44 2
RWS28 GH51 Alpha-l-arabinofuranosidase (Sphingobacterium) WP_038697950.1 100 3E−69 96 33 2
RWS29 GH51 Alpha-l-arabinofuranosidase (Sphingobacterium) WP_045756399.1 100 3E−52 100 43 2
RWS30 GH67 Hemicellulose (alpha-glucuronidase) Alpha-glucuronidase (Sphingobacterium) WP_031288024.1 90 5E−69 83 15 2
RWS31 GH95 Hemicellulose (alpha-fucosidase) Alpha-l-fucosidase (Sphingobacterium) WP_045753034.1 99 8E−72 76 37 4
RWS32 GH95 Alpha-l-fucosidase (Sphingobacterium) WP_045753034.1 100 1E−44 82 46 3
RWS33 GH95 Alpha-l-fucosidase (Sphingobacterium) WP_045753034.1 100 5E−71 77 37 4
RWS34 AA5 Lignin or polysaccharides (glyoxal/galactose oxidases)b Cell envelope biogenesis protein OmpA (Flavobacterium) WP_042565523.1 100 2E−44 99 18 2
RWS35 AA5 Outer membrane protein omp38 (Acinetobacter) WP_004819145.1 66 7E−53 98 39 5
RWS36 AA5 Flagellar motor protein MotB (Sphingobacterium) WP_031288562.1 100 4E−82 98 24 4
RWS37 AA5 Flagellar motor protein MotB (Sphingobacterium) WP_038696344.1 98 4E−53 97 65 6
RWS38 AA5 Membrane protein (Pseudomonas) WP_016485176.1 100 8E−81 100 29 3
  1. COV query coverage, COV2 coverage based on the peptides sizes in each protein, #P number of peptides per protein
  2. aActivities predicted by CAZy database [21, 37]
  3. bCopper radical oxidases able to oxidize carbohydrates, aldehydes, alcohols with generation of H2O2 [37]